Photocontrollable Fluorescent Proteins for Superresolution Imaging

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Photocontrollable fluorescent proteins for superresolution imaging.

Superresolution fluorescence microscopy permits the study of biological processes at scales small enough to visualize fine subcellular structures that are unresolvable by traditional diffraction-limited light microscopy. Many superresolution techniques, including those applicable to live cell imaging, utilize genetically encoded photocontrollable fluorescent proteins. The fluorescence of these ...

متن کامل

Superresolution imaging with fluorescent dyes

With the combination of climate change and human encroachment on the territory of endangered species, information about what and where animals eat and how this is changing over time can help guide conservation efforts. Thure Cerling et al. compiled a 6-year record of nitrogen, carbon, oxygen, and hydrogen in the diet of a family of 4 Kenyan elephants by analyzing the isotopic content of the ele...

متن کامل

Superresolution imaging of multiple fluorescent proteins with highly overlapping emission spectra in living cells.

Localization-based superresolution optical imaging is rapidly gaining popularity, yet limited availability of genetically encoded photoactivatable fluorescent probes with distinct emission spectra impedes simultaneous visualization of multiple molecular species in living cells. We introduce PAmKate, a monomeric photoactivatable far-red fluorescent protein, which facilitates simultaneous imaging...

متن کامل

Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules.

Superresolution imaging techniques based on the precise localization of single molecules, such as photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), achieve high resolution by fitting images of single fluorescent molecules with a theoretical Gaussian to localize them with a precision on the order of tens of nanometers. PALM/STORM rely on phot...

متن کامل

Direct fluorescent-dye labeling of α-tubulin in mammalian cells for live cell and superresolution imaging

Genetic code expansion and bioorthogonal labeling provide for the first time a way for direct, site-specific labeling of proteins with fluorescent-dyes in live cells. Although the small size and superb photophysical parameters of fluorescent-dyes offer unique advantages for high-resolution microscopy, this approach has yet to be embraced as a tool in live cell imaging. Here we evaluated the fea...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Annual Review of Biophysics

سال: 2014

ISSN: 1936-122X,1936-1238

DOI: 10.1146/annurev-biophys-051013-022836